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Psomagen Inc sanger sequencing
Sanger Sequencing, supplied by Psomagen Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sanger+sequencing/sanger+sequencing/pmc08808258-225-2-7
Average 86 stars, based on 1 article reviews
sanger sequencing - by Bioz Stars, 2026-09
86/100 stars

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Related Articles

Polymerase Chain Reaction:

Article Title: Novel Prion Protein Gene ( PRNP) Variants in Wild Montana Mule Deer
Article Snippet: .. Following the PCR amplification, 20μL of the samples were transferred to a 96 well plate for Sanger sequencing by the contract laboratory service provider, Psomagen. .. Sequence data received from Psomagen was analyzed using Geneious Prime (version 2024.0.2) by aligning and comparing with mule deer sequence GenBank accession number AY228473.1 .

Article Title: Haplotype-Based Analysis of OCA2 Variants in Oculocutaneous Albinism.
Article Snippet: TaqMan genotyping (Thermofisher) was performed for variants rs121918166 and rs1800404. .. All other alleles were evaluated by genomic PCR and Sanger sequencing (Psomagen) (Table S1). ..

Article Title: Haplotype‐Based Analysis of OCA2 Variants in Oculocutaneous Albinism
Article Snippet: TaqMan genotyping (Thermofisher) was performed for variants rs121918166 and rs1800404. .. All other alleles were evaluated by genomic PCR and Sanger sequencing (Psomagen) (Table ). ..

Article Title: Molecular investigation of diverse Aedes aegypti in heightened dengue transmission settings in Somaliland, 2023–2024
Article Snippet: .. A subset of PCR amplicons was subjected to Sanger sequencing (Psomagen) to confirm host species. ..

Amplification:

Article Title: Novel Prion Protein Gene ( PRNP) Variants in Wild Montana Mule Deer
Article Snippet: .. Following the PCR amplification, 20μL of the samples were transferred to a 96 well plate for Sanger sequencing by the contract laboratory service provider, Psomagen. .. Sequence data received from Psomagen was analyzed using Geneious Prime (version 2024.0.2) by aligning and comparing with mule deer sequence GenBank accession number AY228473.1 .

Sequencing:

Article Title: Novel Prion Protein Gene ( PRNP) Variants in Wild Montana Mule Deer
Article Snippet: .. Following the PCR amplification, 20μL of the samples were transferred to a 96 well plate for Sanger sequencing by the contract laboratory service provider, Psomagen. .. Sequence data received from Psomagen was analyzed using Geneious Prime (version 2024.0.2) by aligning and comparing with mule deer sequence GenBank accession number AY228473.1 .

Article Title: Haplotype-Based Analysis of OCA2 Variants in Oculocutaneous Albinism.
Article Snippet: TaqMan genotyping (Thermofisher) was performed for variants rs121918166 and rs1800404. .. All other alleles were evaluated by genomic PCR and Sanger sequencing (Psomagen) (Table S1). ..

Article Title: A novel AAA+ ATPase required for sporulation and stress response in Bacillus anthracis .
Article Snippet: The PCR product was cloned into the pPro-Ex-HTc vector using the BamHI and XhoI restriction enzymes (NEB), followed by ligation using T4 DNA ligase (NEB). .. The ligation mixture was transformed into E. coli DH5α, and clones were sequenced using Sanger sequencing (Psomagen). ..

Article Title: A novel AAA+ ATPase required for sporulation and stress response in Bacillus anthracis
Article Snippet: The PCR product was cloned into the pPro-Ex-HTc vector using the BamHI and XhoI restriction enzymes (NEB), followed by ligation using T4 DNA ligase (NEB). .. The ligation mixture was transformed into E. coli DH5α, and clones were sequenced using Sanger sequencing (Psomagen). ..

Article Title: Haplotype‐Based Analysis of OCA2 Variants in Oculocutaneous Albinism
Article Snippet: TaqMan genotyping (Thermofisher) was performed for variants rs121918166 and rs1800404. .. All other alleles were evaluated by genomic PCR and Sanger sequencing (Psomagen) (Table ). ..

Article Title: Molecular investigation of diverse Aedes aegypti in heightened dengue transmission settings in Somaliland, 2023–2024
Article Snippet: .. A subset of PCR amplicons was subjected to Sanger sequencing (Psomagen) to confirm host species. ..

Agarose Gel Electrophoresis:

Article Title: Molecular investigation of diverse Aedes aegypti in heightened dengue transmission settings in Somaliland, 2023–2024
Article Snippet: .. All PCR products were run on a 2% agarose gel for visualization and sent to a commercial laboratory for Sanger Sequencing (Psomagen). .. Aedes aegypti sequence analysis and alignment was conducted with CodonCode (CodonCode Corporation, Centerville, MA, USA).

Ligation:

Article Title: A novel AAA+ ATPase required for sporulation and stress response in Bacillus anthracis .
Article Snippet: The PCR product was cloned into the pPro-Ex-HTc vector using the BamHI and XhoI restriction enzymes (NEB), followed by ligation using T4 DNA ligase (NEB). .. The ligation mixture was transformed into E. coli DH5α, and clones were sequenced using Sanger sequencing (Psomagen). ..

Article Title: A novel AAA+ ATPase required for sporulation and stress response in Bacillus anthracis
Article Snippet: The PCR product was cloned into the pPro-Ex-HTc vector using the BamHI and XhoI restriction enzymes (NEB), followed by ligation using T4 DNA ligase (NEB). .. The ligation mixture was transformed into E. coli DH5α, and clones were sequenced using Sanger sequencing (Psomagen). ..

Transformation Assay:

Article Title: A novel AAA+ ATPase required for sporulation and stress response in Bacillus anthracis .
Article Snippet: The PCR product was cloned into the pPro-Ex-HTc vector using the BamHI and XhoI restriction enzymes (NEB), followed by ligation using T4 DNA ligase (NEB). .. The ligation mixture was transformed into E. coli DH5α, and clones were sequenced using Sanger sequencing (Psomagen). ..

Article Title: A novel AAA+ ATPase required for sporulation and stress response in Bacillus anthracis
Article Snippet: The PCR product was cloned into the pPro-Ex-HTc vector using the BamHI and XhoI restriction enzymes (NEB), followed by ligation using T4 DNA ligase (NEB). .. The ligation mixture was transformed into E. coli DH5α, and clones were sequenced using Sanger sequencing (Psomagen). ..

Clone Assay:

Article Title: A novel AAA+ ATPase required for sporulation and stress response in Bacillus anthracis .
Article Snippet: The PCR product was cloned into the pPro-Ex-HTc vector using the BamHI and XhoI restriction enzymes (NEB), followed by ligation using T4 DNA ligase (NEB). .. The ligation mixture was transformed into E. coli DH5α, and clones were sequenced using Sanger sequencing (Psomagen). ..

Article Title: A novel AAA+ ATPase required for sporulation and stress response in Bacillus anthracis
Article Snippet: The PCR product was cloned into the pPro-Ex-HTc vector using the BamHI and XhoI restriction enzymes (NEB), followed by ligation using T4 DNA ligase (NEB). .. The ligation mixture was transformed into E. coli DH5α, and clones were sequenced using Sanger sequencing (Psomagen). ..



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Mutations in familial OIT. (A) CDHR2 c.2233C > T (p.R745X) was identified by Sanger <t>sequencing.</t> The mutation site is indicated by the red arrow. (B) Sanger sequencing results of individuals from families without CDHR2 nonsense mutations. (C) The genealogy of the OIT lineage in our investigation is depicted using square symbols for males and round symbols for females. The affected individuals are marked in black, and the arrow denotes the proband. Genotypes of BMP15 c.262C > T and CDHR2 c.2233C > T are shown below each symbol.
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Image Search Results


Mutations in familial OIT. (A) CDHR2 c.2233C > T (p.R745X) was identified by Sanger sequencing. The mutation site is indicated by the red arrow. (B) Sanger sequencing results of individuals from families without CDHR2 nonsense mutations. (C) The genealogy of the OIT lineage in our investigation is depicted using square symbols for males and round symbols for females. The affected individuals are marked in black, and the arrow denotes the proband. Genotypes of BMP15 c.262C > T and CDHR2 c.2233C > T are shown below each symbol.

Journal: Human Mutation

Article Title: CDHR2 c.2233C > T Is Involved in Human Familial Ovarian Immature Teratoma With BMP15 c.262C > T

doi: 10.1155/humu/8441244

Figure Lengend Snippet: Mutations in familial OIT. (A) CDHR2 c.2233C > T (p.R745X) was identified by Sanger sequencing. The mutation site is indicated by the red arrow. (B) Sanger sequencing results of individuals from families without CDHR2 nonsense mutations. (C) The genealogy of the OIT lineage in our investigation is depicted using square symbols for males and round symbols for females. The affected individuals are marked in black, and the arrow denotes the proband. Genotypes of BMP15 c.262C > T and CDHR2 c.2233C > T are shown below each symbol.

Article Snippet: Candidate variants were amplified by conventional PCR methods and validated by Sanger sequencing using specific primers by Shanghai Generay Biotech Co., Ltd. ( https://www.generay.com.cn ).

Techniques: Sequencing, Mutagenesis

Diagram of the human CDHR2 gene, protein, and mutant variants. (A) CDHR2 gene structure. The purple and yellow boxes indicate the noncoding domains and exons, respectively. The mutation site is indicated by the red triangle. (B) The primary sequence of the CDHR2 protein and its variant product (p.R745X). The red and green boxes represent extracellular cadherin (EC) repeats and transmembrane (TM) domains of the protein, respectively. PBM indicates the PDZ‐binding motif of CDHR2. A double slash represents a nonsense terminus.

Journal: Human Mutation

Article Title: CDHR2 c.2233C > T Is Involved in Human Familial Ovarian Immature Teratoma With BMP15 c.262C > T

doi: 10.1155/humu/8441244

Figure Lengend Snippet: Diagram of the human CDHR2 gene, protein, and mutant variants. (A) CDHR2 gene structure. The purple and yellow boxes indicate the noncoding domains and exons, respectively. The mutation site is indicated by the red triangle. (B) The primary sequence of the CDHR2 protein and its variant product (p.R745X). The red and green boxes represent extracellular cadherin (EC) repeats and transmembrane (TM) domains of the protein, respectively. PBM indicates the PDZ‐binding motif of CDHR2. A double slash represents a nonsense terminus.

Article Snippet: Candidate variants were amplified by conventional PCR methods and validated by Sanger sequencing using specific primers by Shanghai Generay Biotech Co., Ltd. ( https://www.generay.com.cn ).

Techniques: Mutagenesis, Sequencing, Variant Assay, Binding Assay